@MISC{Moeller2003,
  author = {Moeller, J. and Gottfried, Bj{\"o}rn and Schlieder, Christoph and
	Herzog, Otthein and Friedl, P.},
  title = {{Automated Tracking of Cell Movements and Resolution of Cell-Cell
	Collisions in Three-dimensional Collagen Matrices}},
  howpublished = {Poster presented at the Keystone-Symposium on Cell-Analysis, Breckenridge,
	Colorado},
  year = {2003},
  abstract = {The migration of different cell types such as leukocytes and tumor
	cells within tissues is a fundamental process in physiologic and
	pathologic tissue reactions. However, the investigation and reconstruction
	of cell migration within 3D extracellular matrices in vitro and in
	vivo is limited by the lack of automated quantification methods that
	allow the reconstruction of cell paths from cell populations. We
	have developed a fully automated cell tracking device for the reconstruction
	of migration paths from 3D collagen matrices in front or inhomogeneous
	backgrounds independent of cell size and shape, sharpness of the
	boundary, shape change, and their migration velocity as well as cell-cell
	contacts. Best results for separation of cells from background were
	obtained by region growing from the center of preselected cells until
	a colour gradient was reached. Noise reduction filtering and an object
	matching routine comparing the pixel mass from frame to frame was
	used to optimize segmentation stability upon object shape change
	and background variation, thereby increasing path precision. Overlapping
	paths and cell-cell contacts were resolved by a subtraction algorithm
	using mass changes for path separation. Compared to manual tracking
	as a {"}gold{"} standard automatic tracking resulted in high precision
	and reproducibility of path position, step lengths and frequencies
	(p=0.78 to 0.96; Mann-Whitney U-Test) for migrating ameboid T cells
	as well as tumor cells. This automated device Separating cells with
	identical outlines may be of value for quantitative detection of
	migrating cells in 3D extracellular matrix environments in basic
	research and drug development.},
  owner = {pmania},
  timestamp = {2012.11.06}
}